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Bioconjugate : technique / Greg T. Herm
| Bioconjugate : technique / Greg T. Herm |
| Autore | HERMANSON, Greg T. |
| Pubbl/distr/stampa | San Diego : Academic Press, c1996 |
| Descrizione fisica | XXV, 785 p. : ill. ; 30 cm |
| Disciplina | 574.19 |
| Soggetto non controllato |
Biochimica
Proteine Acidi nucleici |
| ISBN | 0-12-342336-8 |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNISA-990000313820203316 |
HERMANSON, Greg T.
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| San Diego : Academic Press, c1996 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. di Salerno | ||
| ||
Bioconjugate techniques / Greg T. Hermanson
| Bioconjugate techniques / Greg T. Hermanson |
| Autore | Hermanson, Greg T. |
| Edizione | [Third edition.] |
| Pubbl/distr/stampa | London ; Waltham, MA : Elsevier/AP, 2013 |
| Descrizione fisica | XVII, 1146 p. : ill. ; 28 cm |
| Disciplina | 612 |
| Soggetto non controllato | Biosintesi |
| ISBN | 9780123822390 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-990009924520403321 |
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Hermanson, Greg T.
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| London ; Waltham, MA : Elsevier/AP, 2013 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Bioconjugate techniques [[electronic resource]] / Greg T. Hermanson
| Bioconjugate techniques [[electronic resource]] / Greg T. Hermanson |
| Autore | Hermanson Greg T |
| Edizione | [3rd ed.] |
| Pubbl/distr/stampa | London, : Academic Press, c2013 |
| Descrizione fisica | 1 online resource (1165 p.) |
| Disciplina | 574.19/296 |
| Soggetto topico | Bioconjugates - Synthesis |
| Soggetto genere / forma | Electronic books. |
| ISBN | 0-12-382240-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Bioconjugate Techniques; Copyright Page; Dedication; Contents; Preface to the Third Edition; Acknowledgments; Health and Safety; Intellectual Property; Important Information; 1. Introduction to Bioconjugation; 1. What is Bioconjugation?; 2. Bioconjugation Strategy and Design; 2.1. Start with the Application in Mind; 2.2. Designing the Optimal Bioconjugate; 3. The Applications of Bioconjugates; 3.1. Assay and Quantification; Bioconjugates Used in Heterogeneous Immunoassays; Bioconjugates for Enzyme-Linked Immunoassays; Chemiluminescent Acridinium Ester Conjugates
Fluorescent Oligonucleotide ProbesMolecular Scaffolds to Increase Assay Sensitivity; Bioconjugates Used in Homogeneous Assays; Bioconjugates for FRET Assays; Molecular Beacons and TaqMan Probes; Bioluminescence Resonance Energy Transfer (BRET); Protein Fragment Complementation Assays: Split Reporters; Mass Tags for Quantitative Mass Spectrometry; Limiting Nonspecificity in Bioconjugate Designs; 3.2. Detection, Tracking, and Imaging; Fluorescently Labeled Antibodies and Streptavidin; FRET Based Protease Probes; Interacting Proteins or Domains; Probing by Chemoselective Ligation Proximity Ligation AssaysStaining with Antibody-Enzyme Conjugates; Bioconjugates for Western Blot Detection; Bioconjugates for Super Resolution Microscopy; 3.3. Purification, Capture, and Scavenging; Purification using Immobilized Affinity Ligands; Immunoprecipitation Techniques; Affinity Capture of Post-Translational Modifications; Affinity Capture using Active Site Binding Probes; Affinity Capture of Recombinant Fusion Proteins; Covalent Fusion Tag Technology; Scavenging of Contaminants or Unwanted Components; 3.4. Catalysis and Chemical Modification Immobilized Proteases for Proteomic AnalysisImmobilized Reactors in Bioengineering; 3.5. Therapeutics and in vivo Diagnostics; Bioconjugates for Cancer Therapy; Antibody Targeting for Biotherapeutics; Polymeric Scaffolds and Nanoparticles for Biotherapeutic Conjugates; Antibody-Directed Enzyme Prodrug Therapy; Radiolabeled Bioconjugates for Cancer; Boron Neutron Capture Therapy; Photodynamic Therapy; Diagnostic Bioconjugates for In Vivo Imaging; Bioconjugates for Radio Imaging; Bioconjugates for High-Contrast Imaging; NIR Fluorescent Conjugates for In Vivo Imaging 3.6. Vaccines and Immune ModulationCancer Vaccines; Immunogen Conjugates in the Production of Antibodies; 4. Summary; 2. Functional Targets for Bioconjugation; 1. Modification of Amino Acids, Peptides, and Proteins; 1.1. Protein Structure and Reactivity; Amino Acids; Nucleophilic Reactions and the pI of Amino Acid Side Chains; Secondary, Tertiary, and Quaternary Structure; Prosthetic Groups, Cofactors, and Post-Translational Modifications; Protecting the Native Conformation and Activity of Proteins; Oxidation of Amino Acids in Proteins and Peptides Solvent Accessibility of Functional Targets in Proteins |
| Record Nr. | UNINA-9910452672903321 |
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Hermanson Greg T
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| London, : Academic Press, c2013 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
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Bioconjugate techniques / / Greg T. Hermanson, Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL
| Bioconjugate techniques / / Greg T. Hermanson, Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL |
| Autore | Hermanson Greg T |
| Edizione | [3rd ed.] |
| Pubbl/distr/stampa | London, : Academic Press, c2013 |
| Descrizione fisica | 1 online resource (xvii, 1146 pages) : illustrations (some color) |
| Disciplina | 574.19/296 |
| Collana | Gale eBooks |
| Soggetto topico | Bioconjugates - Synthesis |
| ISBN | 0-12-382240-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Bioconjugate Techniques; Copyright Page; Dedication; Contents; Preface to the Third Edition; Acknowledgments; Health and Safety; Intellectual Property; Important Information; 1. Introduction to Bioconjugation; 1. What is Bioconjugation?; 2. Bioconjugation Strategy and Design; 2.1. Start with the Application in Mind; 2.2. Designing the Optimal Bioconjugate; 3. The Applications of Bioconjugates; 3.1. Assay and Quantification; Bioconjugates Used in Heterogeneous Immunoassays; Bioconjugates for Enzyme-Linked Immunoassays; Chemiluminescent Acridinium Ester Conjugates
Fluorescent Oligonucleotide ProbesMolecular Scaffolds to Increase Assay Sensitivity; Bioconjugates Used in Homogeneous Assays; Bioconjugates for FRET Assays; Molecular Beacons and TaqMan Probes; Bioluminescence Resonance Energy Transfer (BRET); Protein Fragment Complementation Assays: Split Reporters; Mass Tags for Quantitative Mass Spectrometry; Limiting Nonspecificity in Bioconjugate Designs; 3.2. Detection, Tracking, and Imaging; Fluorescently Labeled Antibodies and Streptavidin; FRET Based Protease Probes; Interacting Proteins or Domains; Probing by Chemoselective Ligation Proximity Ligation AssaysStaining with Antibody-Enzyme Conjugates; Bioconjugates for Western Blot Detection; Bioconjugates for Super Resolution Microscopy; 3.3. Purification, Capture, and Scavenging; Purification using Immobilized Affinity Ligands; Immunoprecipitation Techniques; Affinity Capture of Post-Translational Modifications; Affinity Capture using Active Site Binding Probes; Affinity Capture of Recombinant Fusion Proteins; Covalent Fusion Tag Technology; Scavenging of Contaminants or Unwanted Components; 3.4. Catalysis and Chemical Modification Immobilized Proteases for Proteomic AnalysisImmobilized Reactors in Bioengineering; 3.5. Therapeutics and in vivo Diagnostics; Bioconjugates for Cancer Therapy; Antibody Targeting for Biotherapeutics; Polymeric Scaffolds and Nanoparticles for Biotherapeutic Conjugates; Antibody-Directed Enzyme Prodrug Therapy; Radiolabeled Bioconjugates for Cancer; Boron Neutron Capture Therapy; Photodynamic Therapy; Diagnostic Bioconjugates for In Vivo Imaging; Bioconjugates for Radio Imaging; Bioconjugates for High-Contrast Imaging; NIR Fluorescent Conjugates for In Vivo Imaging 3.6. Vaccines and Immune ModulationCancer Vaccines; Immunogen Conjugates in the Production of Antibodies; 4. Summary; 2. Functional Targets for Bioconjugation; 1. Modification of Amino Acids, Peptides, and Proteins; 1.1. Protein Structure and Reactivity; Amino Acids; Nucleophilic Reactions and the pI of Amino Acid Side Chains; Secondary, Tertiary, and Quaternary Structure; Prosthetic Groups, Cofactors, and Post-Translational Modifications; Protecting the Native Conformation and Activity of Proteins; Oxidation of Amino Acids in Proteins and Peptides Solvent Accessibility of Functional Targets in Proteins |
| Record Nr. | UNINA-9910779993503321 |
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Hermanson Greg T
|
||
| London, : Academic Press, c2013 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Bioconjugate techniques / / Greg T. Hermanson, Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL
| Bioconjugate techniques / / Greg T. Hermanson, Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL |
| Autore | Hermanson Greg T |
| Edizione | [3rd ed.] |
| Pubbl/distr/stampa | London, : Academic Press, c2013 |
| Descrizione fisica | 1 online resource (xvii, 1146 pages) : illustrations (some color) |
| Disciplina | 574.19/296 |
| Collana | Gale eBooks |
| Soggetto topico | Bioconjugates - Synthesis |
| ISBN | 0-12-382240-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Bioconjugate Techniques; Copyright Page; Dedication; Contents; Preface to the Third Edition; Acknowledgments; Health and Safety; Intellectual Property; Important Information; 1. Introduction to Bioconjugation; 1. What is Bioconjugation?; 2. Bioconjugation Strategy and Design; 2.1. Start with the Application in Mind; 2.2. Designing the Optimal Bioconjugate; 3. The Applications of Bioconjugates; 3.1. Assay and Quantification; Bioconjugates Used in Heterogeneous Immunoassays; Bioconjugates for Enzyme-Linked Immunoassays; Chemiluminescent Acridinium Ester Conjugates
Fluorescent Oligonucleotide ProbesMolecular Scaffolds to Increase Assay Sensitivity; Bioconjugates Used in Homogeneous Assays; Bioconjugates for FRET Assays; Molecular Beacons and TaqMan Probes; Bioluminescence Resonance Energy Transfer (BRET); Protein Fragment Complementation Assays: Split Reporters; Mass Tags for Quantitative Mass Spectrometry; Limiting Nonspecificity in Bioconjugate Designs; 3.2. Detection, Tracking, and Imaging; Fluorescently Labeled Antibodies and Streptavidin; FRET Based Protease Probes; Interacting Proteins or Domains; Probing by Chemoselective Ligation Proximity Ligation AssaysStaining with Antibody-Enzyme Conjugates; Bioconjugates for Western Blot Detection; Bioconjugates for Super Resolution Microscopy; 3.3. Purification, Capture, and Scavenging; Purification using Immobilized Affinity Ligands; Immunoprecipitation Techniques; Affinity Capture of Post-Translational Modifications; Affinity Capture using Active Site Binding Probes; Affinity Capture of Recombinant Fusion Proteins; Covalent Fusion Tag Technology; Scavenging of Contaminants or Unwanted Components; 3.4. Catalysis and Chemical Modification Immobilized Proteases for Proteomic AnalysisImmobilized Reactors in Bioengineering; 3.5. Therapeutics and in vivo Diagnostics; Bioconjugates for Cancer Therapy; Antibody Targeting for Biotherapeutics; Polymeric Scaffolds and Nanoparticles for Biotherapeutic Conjugates; Antibody-Directed Enzyme Prodrug Therapy; Radiolabeled Bioconjugates for Cancer; Boron Neutron Capture Therapy; Photodynamic Therapy; Diagnostic Bioconjugates for In Vivo Imaging; Bioconjugates for Radio Imaging; Bioconjugates for High-Contrast Imaging; NIR Fluorescent Conjugates for In Vivo Imaging 3.6. Vaccines and Immune ModulationCancer Vaccines; Immunogen Conjugates in the Production of Antibodies; 4. Summary; 2. Functional Targets for Bioconjugation; 1. Modification of Amino Acids, Peptides, and Proteins; 1.1. Protein Structure and Reactivity; Amino Acids; Nucleophilic Reactions and the pI of Amino Acid Side Chains; Secondary, Tertiary, and Quaternary Structure; Prosthetic Groups, Cofactors, and Post-Translational Modifications; Protecting the Native Conformation and Activity of Proteins; Oxidation of Amino Acids in Proteins and Peptides Solvent Accessibility of Functional Targets in Proteins |
| Record Nr. | UNINA-9910827166303321 |
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Hermanson Greg T
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||
| London, : Academic Press, c2013 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Bioconjugate techniques / Greg T. Hermanson
| Bioconjugate techniques / Greg T. Hermanson |
| Autore | Hermanson, Greg T. |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | Amsterdam [etc.] : Elsevier, 2008 |
| Descrizione fisica | X, 1202 p. : ill. ; 24 cm |
| Disciplina | 572 |
| Soggetto non controllato | Biosintesi |
| ISBN | 9780123705013 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-990009394810403321 |
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Hermanson, Greg T.
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| Amsterdam [etc.] : Elsevier, 2008 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Bioconjugate techniques [[electronic resource] /] / Greg T. Hermanson
| Bioconjugate techniques [[electronic resource] /] / Greg T. Hermanson |
| Autore | Hermanson Greg T |
| Pubbl/distr/stampa | San Diego, : Academic Press, 1996 |
| Descrizione fisica | 1 online resource (813 p.) |
| Disciplina | 574.19/296 |
| Soggetto topico |
Bioconjugates - Synthesis
Biochemistry |
| Soggetto genere / forma | Electronic books. |
| ISBN |
0-08-057212-X
1-281-01960-7 9786611019600 0-08-052790-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Bioconjugate Techniques; Copyright Page; Detailed Contents; Preface; Acknowledgments; Part I: Bioconjugate Chemistry; Chapter 1. Functional Targets; 1. Modification of Amino Acids, Peptides, and Proteins; 2. Modification of Sugars, Polysaccharides, and Glycoconjugates; 3. Modification of Nucleic Acids and Oligonucleotides; 4. Creating Specific Functional Groups; 5. Blocking Specific Functional Groups; Chapter 2. The Chemistry of Reactive Groups; 1. Amine-Reactive Chemical Reactions; 2. Thiol-Reactive Chemical Reactions; 3. Carboxylate-Reactive Chemical Reactions
4. Hydroxyl-Reactive Chemical Reactions5. Aldehyde- and Ketone-Reactive Chemical Reactions; 6. Active Hydrogen-Reactive Chemical Reactions; 7. Photoreactive Chemical Reactions; Part II: Bioconjugate Reagents; Chapter 3. Zero-Length Cross-linkers; 1. Carbodiimides; 2. Woodward's Reagent K; 3. N, N'-Carbonyldiimidazole; 4. Schiff Base Formation and Reductive Amination; Chapter 4. Homobifunctional Cross-linkers; 1. Homobifunctional NHS Esters; 2. Homobifunctional Imidoesters; 3. Homobifunctional Sulfhydryl-Reactive Cross-linkers; 4. Difluorobenzene Derivatives 5. Homobifunctional Photoreactive Cross-linkers6. Homobifunctional Aldehydes; 7. Bis-epoxides; 8. Homobifuncrional Hydrazides; 9. Bis-diazonium Derivatives; 10. Bis-alkylhalides; Chapter 5. Heterobifunctional Cross-linkers; 1. Amine-Reactive and Sulfhydryl-Reactive Cross-linkers; 2. Carbonyl-Reactive and Sulfhydryl-Reactive Cross-linkers; 3. Amine-Reactive and Photoreactive Cross-linkers; 4. Sulfhydryl-Reactive and Photoreactive Cross-linkers; 5. Carbonyl-Reactive and Photoreactive Cross-linkers; 6. Carboxylate-Reactive and Photoreactive Cross-linkers 7. Arginine-Reactive and Photoreactive Cross-linkersChapter 6. Trifunctional Cross-linkers; 1. 4-Azido-2-nitrophenylbiocytin-4-nitrophenyl ester; 2. Sulfo-SBED; Chapter 7. Cleavable Reagent Systems; 1. Cleavage of Disulfides by Reduction; 2. Periodate-Cleavable Glycols; 3. Dithionite-Cleavable Diazo Bonds; 4. Hydroxylamine-Cleavable Esters; 5. Base Labile Sulfones; Chapter 8. Tags and Probes; 1. Fluorescent Labels; 2. Bifunctional Chelating Agents and Radioimmunoconjugates; 3. Biotinylation Reagents; 4. lodination Reagents; Part III: Bioconjugate Applications Chapter 9. Preparation of Hapten-Carrier Immunogen Conjugates1. The Basis of Immunity; 2. Types of Immunogen Carriers; 3. Carbodiimide-Mediated Hapten-Carrier Conjugation; 4. NHS Ester-Mediated Hapten-Carrier Conjugation; 5. NHS Ester-Maleimide Heterobifunctional Cross-linker- Mediated Hapten-Carrier Conjugation; 6. Active-Hydrogen-Mediated Hapten-Carrier Conjugation; 7. Glutaraldehyde-Mediated Hapten-Carrier Conjugation; 8. Reductive-Amination-Mediated Hapten-Carrier Conjugation; Chapter 10. Antibody Modification and Conjugation; 1. Preparation of Antibody-Enzyme Conjugates 2. Preparation of Labeled Antibodies |
| Record Nr. | UNINA-9910457975803321 |
|
Hermanson Greg T
|
||
| San Diego, : Academic Press, 1996 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Bioconjugate techniques [[electronic resource] /] / Greg T. Hermanson
| Bioconjugate techniques [[electronic resource] /] / Greg T. Hermanson |
| Autore | Hermanson Greg T |
| Pubbl/distr/stampa | San Diego, : Academic Press, 1996 |
| Descrizione fisica | 1 online resource (813 p.) |
| Disciplina | 574.19/296 |
| Soggetto topico |
Bioconjugates - Synthesis
Biochemistry |
| ISBN |
0-08-057212-X
1-281-01960-7 9786611019600 0-08-052790-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Bioconjugate Techniques; Copyright Page; Detailed Contents; Preface; Acknowledgments; Part I: Bioconjugate Chemistry; Chapter 1. Functional Targets; 1. Modification of Amino Acids, Peptides, and Proteins; 2. Modification of Sugars, Polysaccharides, and Glycoconjugates; 3. Modification of Nucleic Acids and Oligonucleotides; 4. Creating Specific Functional Groups; 5. Blocking Specific Functional Groups; Chapter 2. The Chemistry of Reactive Groups; 1. Amine-Reactive Chemical Reactions; 2. Thiol-Reactive Chemical Reactions; 3. Carboxylate-Reactive Chemical Reactions
4. Hydroxyl-Reactive Chemical Reactions5. Aldehyde- and Ketone-Reactive Chemical Reactions; 6. Active Hydrogen-Reactive Chemical Reactions; 7. Photoreactive Chemical Reactions; Part II: Bioconjugate Reagents; Chapter 3. Zero-Length Cross-linkers; 1. Carbodiimides; 2. Woodward's Reagent K; 3. N, N'-Carbonyldiimidazole; 4. Schiff Base Formation and Reductive Amination; Chapter 4. Homobifunctional Cross-linkers; 1. Homobifunctional NHS Esters; 2. Homobifunctional Imidoesters; 3. Homobifunctional Sulfhydryl-Reactive Cross-linkers; 4. Difluorobenzene Derivatives 5. Homobifunctional Photoreactive Cross-linkers6. Homobifunctional Aldehydes; 7. Bis-epoxides; 8. Homobifuncrional Hydrazides; 9. Bis-diazonium Derivatives; 10. Bis-alkylhalides; Chapter 5. Heterobifunctional Cross-linkers; 1. Amine-Reactive and Sulfhydryl-Reactive Cross-linkers; 2. Carbonyl-Reactive and Sulfhydryl-Reactive Cross-linkers; 3. Amine-Reactive and Photoreactive Cross-linkers; 4. Sulfhydryl-Reactive and Photoreactive Cross-linkers; 5. Carbonyl-Reactive and Photoreactive Cross-linkers; 6. Carboxylate-Reactive and Photoreactive Cross-linkers 7. Arginine-Reactive and Photoreactive Cross-linkersChapter 6. Trifunctional Cross-linkers; 1. 4-Azido-2-nitrophenylbiocytin-4-nitrophenyl ester; 2. Sulfo-SBED; Chapter 7. Cleavable Reagent Systems; 1. Cleavage of Disulfides by Reduction; 2. Periodate-Cleavable Glycols; 3. Dithionite-Cleavable Diazo Bonds; 4. Hydroxylamine-Cleavable Esters; 5. Base Labile Sulfones; Chapter 8. Tags and Probes; 1. Fluorescent Labels; 2. Bifunctional Chelating Agents and Radioimmunoconjugates; 3. Biotinylation Reagents; 4. lodination Reagents; Part III: Bioconjugate Applications Chapter 9. Preparation of Hapten-Carrier Immunogen Conjugates1. The Basis of Immunity; 2. Types of Immunogen Carriers; 3. Carbodiimide-Mediated Hapten-Carrier Conjugation; 4. NHS Ester-Mediated Hapten-Carrier Conjugation; 5. NHS Ester-Maleimide Heterobifunctional Cross-linker- Mediated Hapten-Carrier Conjugation; 6. Active-Hydrogen-Mediated Hapten-Carrier Conjugation; 7. Glutaraldehyde-Mediated Hapten-Carrier Conjugation; 8. Reductive-Amination-Mediated Hapten-Carrier Conjugation; Chapter 10. Antibody Modification and Conjugation; 1. Preparation of Antibody-Enzyme Conjugates 2. Preparation of Labeled Antibodies |
| Record Nr. | UNINA-9910784528803321 |
|
Hermanson Greg T
|
||
| San Diego, : Academic Press, 1996 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Bioconjugate techniques [[electronic resource] /] / Greg T. Hermanson
| Bioconjugate techniques [[electronic resource] /] / Greg T. Hermanson |
| Autore | Hermanson Greg T |
| Pubbl/distr/stampa | San Diego, : Academic Press, 1996 |
| Descrizione fisica | 1 online resource (813 p.) |
| Disciplina | 574.19/296 |
| Soggetto topico |
Bioconjugates - Synthesis
Biochemistry |
| ISBN |
0-08-057212-X
1-281-01960-7 9786611019600 0-08-052790-6 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Bioconjugate Techniques; Copyright Page; Detailed Contents; Preface; Acknowledgments; Part I: Bioconjugate Chemistry; Chapter 1. Functional Targets; 1. Modification of Amino Acids, Peptides, and Proteins; 2. Modification of Sugars, Polysaccharides, and Glycoconjugates; 3. Modification of Nucleic Acids and Oligonucleotides; 4. Creating Specific Functional Groups; 5. Blocking Specific Functional Groups; Chapter 2. The Chemistry of Reactive Groups; 1. Amine-Reactive Chemical Reactions; 2. Thiol-Reactive Chemical Reactions; 3. Carboxylate-Reactive Chemical Reactions
4. Hydroxyl-Reactive Chemical Reactions5. Aldehyde- and Ketone-Reactive Chemical Reactions; 6. Active Hydrogen-Reactive Chemical Reactions; 7. Photoreactive Chemical Reactions; Part II: Bioconjugate Reagents; Chapter 3. Zero-Length Cross-linkers; 1. Carbodiimides; 2. Woodward's Reagent K; 3. N, N'-Carbonyldiimidazole; 4. Schiff Base Formation and Reductive Amination; Chapter 4. Homobifunctional Cross-linkers; 1. Homobifunctional NHS Esters; 2. Homobifunctional Imidoesters; 3. Homobifunctional Sulfhydryl-Reactive Cross-linkers; 4. Difluorobenzene Derivatives 5. Homobifunctional Photoreactive Cross-linkers6. Homobifunctional Aldehydes; 7. Bis-epoxides; 8. Homobifuncrional Hydrazides; 9. Bis-diazonium Derivatives; 10. Bis-alkylhalides; Chapter 5. Heterobifunctional Cross-linkers; 1. Amine-Reactive and Sulfhydryl-Reactive Cross-linkers; 2. Carbonyl-Reactive and Sulfhydryl-Reactive Cross-linkers; 3. Amine-Reactive and Photoreactive Cross-linkers; 4. Sulfhydryl-Reactive and Photoreactive Cross-linkers; 5. Carbonyl-Reactive and Photoreactive Cross-linkers; 6. Carboxylate-Reactive and Photoreactive Cross-linkers 7. Arginine-Reactive and Photoreactive Cross-linkersChapter 6. Trifunctional Cross-linkers; 1. 4-Azido-2-nitrophenylbiocytin-4-nitrophenyl ester; 2. Sulfo-SBED; Chapter 7. Cleavable Reagent Systems; 1. Cleavage of Disulfides by Reduction; 2. Periodate-Cleavable Glycols; 3. Dithionite-Cleavable Diazo Bonds; 4. Hydroxylamine-Cleavable Esters; 5. Base Labile Sulfones; Chapter 8. Tags and Probes; 1. Fluorescent Labels; 2. Bifunctional Chelating Agents and Radioimmunoconjugates; 3. Biotinylation Reagents; 4. lodination Reagents; Part III: Bioconjugate Applications Chapter 9. Preparation of Hapten-Carrier Immunogen Conjugates1. The Basis of Immunity; 2. Types of Immunogen Carriers; 3. Carbodiimide-Mediated Hapten-Carrier Conjugation; 4. NHS Ester-Mediated Hapten-Carrier Conjugation; 5. NHS Ester-Maleimide Heterobifunctional Cross-linker- Mediated Hapten-Carrier Conjugation; 6. Active-Hydrogen-Mediated Hapten-Carrier Conjugation; 7. Glutaraldehyde-Mediated Hapten-Carrier Conjugation; 8. Reductive-Amination-Mediated Hapten-Carrier Conjugation; Chapter 10. Antibody Modification and Conjugation; 1. Preparation of Antibody-Enzyme Conjugates 2. Preparation of Labeled Antibodies |
| Record Nr. | UNINA-9910817141803321 |
|
Hermanson Greg T
|
||
| San Diego, : Academic Press, 1996 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Bioconjugate techniques / Greg T. Hermanson
| Bioconjugate techniques / Greg T. Hermanson |
| Autore | Hermanson, Greg T. |
| Edizione | [3rd ed] |
| Descrizione fisica | xvii, 1146 pages : illustrations ; 28 cm |
| Disciplina | 612.1111 |
| Soggetto topico |
Bioconjugates - Synthesis
Biomolecules - Researches - Diagnostics - Therapeutics |
| ISBN | 9780123822390 (hbk) |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNISALENTO-991003689769707536 |
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Hermanson, Greg T.
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| Materiale a stampa | ||
| Lo trovi qui: Univ. del Salento | ||
| ||
